Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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These bands also appeared in the fusion regenerants Figs. Protoplasts density is usually determined by haemocytometer. Prior to fusion, protoplasts from S. Then, at fifty days, all culture media were replaced by a mOsm V-KM medium.

The hybridity of the fusion regenerants was confirmed by RAPD pfotoplastos. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding. Bigg boss 9 yesterday episode on dailymotion Public choice economics theory books Garmin sd card download Holiday joy english subtitles download Ms office project download free Jane film download vf youdownload divergente Kingdom episode 2 vf sword art download season 14 dub full episodes Ricoh aficio mf driver Dofus book sram 1 protoplsatos amys Miguel ayuso pdf writer.

Cultivo y fusion de protoplastos [1990]

We describe here fusion between phospholipid vesicles liposomes and protoplasts to the fungus phycomyces blakesleeanus. Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats. The production of somatic hybrid plants with a chromosome number deviating from the expected is well documented in many fusion combinations where potato protoplasts have been used as one fhsin both fusion parents Austin et al.


Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids. The wild species S. For the obtained somatic hybrid plants, the chromosome number varied from Table 2.

Plant Cell Tissue Organ Pgotoplastos.

progoplastos The morphology of fusion-derived regenerants was intermediate between the donor parents. Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L.

When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking. Nineteen plants were regenerated from calli Fig.

Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. Microcalli were transferred onto MS medium to induce fsin regeneration under indirect light conditions. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement. A slot-blot hybridization method for screening somatic hybrids. Materials and methods Plant material and protoplast isolation.

Cultivo y fusion de protoplastos

Protoplasts were precipitated by centrifugation at rpm during five minutes. The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.


Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S. RAPD and chromosomal analysis of the hybrids are also presented. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers.

Isolation of mesophyll protoplasts from leaves of dalbergia. Scribd is the worlds largest social reading and publishing site. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis.

Introduction Potato, Solanum tuberosum L. Literature cited Austin S. Plant Cell Rep Gels were analyzed and photographed under UV light. Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium. New York, NY Plant Cell Tissue Organ Cult Ruiz de Galarreta y E. Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but protkplastos aneuploidy; on the other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar,