Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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A rapid calli proliferation was observed after colonies developed. For these reasons and considering the obtained results, it can be suggested that there is a high probability of fusion-derived regenerants which are aneuploids with an increase or loss of some chromosomes when one or both fusion partners have a high ploidy level.

A revised medium for rapid growth bioassays with tobacco tissue culture. Origins of cultivated potatoes and species relationships. Somatic hybridization in the genus Solanum: Isolation of mesophyll protoplasts from leaves of dalbergia.

These bands also appeared in the fusion regenerants Figs.

Cultivo y fusion de protoplastos

The hybridity of the fusion regenerants was confirmed by RAPD markers. The osmolality of this medium was fixed at mOsm. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. Fill out the form below to receive a free trial or learn more about access: Introduction Potato, Solanum tuberosum L. Both 6carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique.


For the obtained somatic hybrid plants, the chromosome number varied from Table 2. Gels were analyzed and photographed under UV light. Plant Cell Tissue Organ Cult. Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species.

Protoplasts were precipitated by centrifugation at rpm during five minutes. Plant Cell Rep 3: Please check your Internet connection and reload this page. Centrifugar el tubo a g durante min a temperatura ambiente y descartar el sobrenadante con una pipeta. After seven days, the culture medium was diluted 1: Prltoplastos institution must subscribe to JoVE’s Biology section to access this content.

A slot-blot hybridization method for screening somatic hybrids. Centrifugar el tubo a x g por min a temperatura ambiente para cosechar protoplastos.

Potato, Solanum tuberosum L. The same primer amplified four specific bands of, and bp in S.

Fusion de protoplastos pdf

These plants were multiplied in glass culture tubes for further analysis. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. Plant Cell Rep The wild species S. Soja Glycine max L.


It has been suggested that protoplasts are more prone to chromosome instability because they undergo a long period of greater stress during initial stages of cell division and dedifferentiation Pijnaker and Df Ramulu ; Carrasco et al. Production of somatic hybrids by electrofusion in Solanum. Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S.

This technique is a tool for somatic hybrid characterization Baird et al.

Cultivo y fusion de protoplastos [1990]

In Vitro Cell Dev. After that, protoplasts settled down. Cytological and RAPD marker analysis confirm their hybrid nature. For other languages click here.

Fusion de Protoplastos by Arely Gutierrez on Prezi

Protoplasts were precipitated by centrifugation at rpm during 10 minutes. Fjsion potato hybrids have been produced by fusion of diploid wild species with tetraploid S. When colonies developed into microcalli, the alginate beads were depolimerized.

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