INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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Studies on inulinases produced by some bacteria. Production of inulin fructotransferase depolymerizing by Artrobacter sp. Highly active isolate of Streptomyces grisenus isolated from soil sample and identified according to Cause et al.

Besides, various promising applications and inluinase of enzymatic hydrolysis for producing important industrial products also have been discussed. Purification and Characterization of Exoinulinase Enzyme from Purofication grisenus. Purification of the extra cellular inulinase: However, to expand the utility of inulinases in various industries, an extensive investigation for novel microbial sources should be conducted and low cost substrates, and improved bioprocessing technology should be used.

Inulin can be considered as dietary fibre, a substitute for fat and low calorie sweetener Roberfroid, Therefore, this study aimed to collate all information, particularly on fungal sources, characteristics, and applications of inulinases. Factors affecting purified inulinase enzyme: Oligosaccharides are compounds with great potential of use in food industry particularly interesting are fructooligosaccharids FOSbecause of their favorable functional properties such as low calorie and noncariogenic sweeteners, improvement of the intestinal microbial flora, relief of constipation, decrease of total cholesterol and lipid in the serum and promotion of animal growth Vandamme and Derycke, The optimum growth and inulinase activity occurred in the presence of 0.

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Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

Biochemical, biophysical properties and structure-function analysis elucidates the substrate specificity of enzyme as well as purifucation phylogenetic evolution and relationship. The most potent isolate was identified as Streptomyces grisenus. Data in Table 9 revealed the effect of metal ions on the activity of purified inulinase in the enzyme mixture.

The results in Table 6 and 7 revealed the effect of some physical conditions on purified enzyme produced by streptomyces grisenus viz. Inulin was the best carbon source for inulinase production, while starch, maltose and lactose were inadequate. Elsevier Applied Science, London, pp: Hydrolysis of inulin from jerusalem artichake by inulinase immobilized on aminoethyl cellulose enzyme. Close Print this page.

Effect of different substrate concentration on the purified inulinase produced by Streptomyces grisenus. The progress in inulinase production under submerged and solid-state fermentation is presented along with the description of innovations used in the production of recombinant inulinases. Its production, properties and industrial applications.

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In the present review, various properties of inulin as a potent substrate for inulinase production are summarized. How to cite this article: Present findings are in agreement with Lim et al. Purification, properties and comparison of invertase, exoinulinase and endoinulinase of Aspergillus ficuum. The fermentation products from these groups of bacteria give rise to local and systemic health effects.

Production, Purification, Characterization and Applications of Fungal Inulinases

Inulin, inulinases, purification, high fructose syrup, fructooligosaccharides, enzymes. Pakistan Journal of Biological Sciences, 9: Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. Elution was carried out by 0.

Effect of temperature on the inulinase activity of Streptomyces grisenus. Purification and characterizationof an endoinulinase from Xanthomonas oryzae No. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Effect of different pH values on the inulinase activity of Streptomyces grisenus.